HPLC method for simultaneous determination of enantiomers of 5-dimethylsulphamoyl-6,7-dichloro-2,3-dihydrobenzofuran-2-carboxylic acid and its N-monodemethyl metabolite in monkey plasma and urine afte
Summary
Type: HPLC Application #: 105640
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 223nm for plasma, 254nm for urine Conditions: eluent: acetonitrile - methanol - water (45:5:50) for plasma eluent: acetonitrile - 2-propanol - water (40:5:55) for urine flow rate 1ml/min Authors: Nakano M Source: J Chromatogr 564 (1991) 235 - 241
Details
Legend: Fig. 2. Chromatograms of S-(-)-a-methylbenzylamide derivatized biological
samples, a) and d) blank plasma and urine, b) blank plasma spiked with 0.5µg of
each enantiomer, e) blank urine spiked with